Moesin Recombinant antibody, PBS Only

• Featured Product
• KD/KO Validated

Moesin Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3B8

Cat no : 82009-1-PBS

Synonyms

MSN, Membrane-organizing extension spike protein, 3B8

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Validation Data Gallery

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  WB analysis using 82009-1-RR (same clone as 82009-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82009-1-RR (MSN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82009-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 82009-1-RR (same clone as 82009-1-PBS)

  WB result of Moesin antibody (82009-1-RR; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Moesin transfected HeLa cells. This data was developed using the same antibody clone with 82009-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 82009-1-RR (same clone as 82009-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82009-1-RR (Moesin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82009-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 82009-1-RR (same clone as 82009-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82009-1-RR (Moesin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82009-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 82009-1-RR (same clone as 82009-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82009-1-RR (Moesin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82009-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 82009-1-RR (same clone as 82009-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Moesin antibody (82009-1-RR, Clone: 3B8 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82009-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82009-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82009-1-RR against Human MSN were performed. The affinity constant is below 1 pM.

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Product Information

82009-1-PBS targets Moesin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Moesin fusion protein Ag9623
• Full Name: moesin
• Calculated Molecular Weight: 577 aa, 68 kDa
• Observed Molecular Weight: 68-70 kDa
• GenBank Accession Number: BC017293
• Gene Symbol: Moesin
• Gene ID (NCBI): 4478
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Moesin belongs to the ezrin-radixin-moesin (ERM) family of proteins which act as cross-linkers between membrane and actin cytoskeleton. ERM proteins provide structural links to strengthen the cell cortex and facilitate several key cellular processes, including membrane dynamics, substrate adhesion, cell survival, cell adhesion, and motility. The function of ERM proteins is highly reliant on phosphorylation induced conformational changes in response to growth factor, chemokine, and antigen stimulation. This antibody may cross-react with ezrin or radixin with molecular weights around 68-70 kDa.