Methylmalonyl Coenzyme A mutase/MUT Recombinant antibody, PBS Only

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  WB analysis using 84879-5-RR (same clone as 84879-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84879-5-RR (Methylmalonyl Coenzyme A mutase/MUT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84879-5-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 84879-5-RR (same clone as 84879-5-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug Methylmalonyl Coenzyme A mutase/MUT Recombinant antibody (84879-5-RR, Clone:242198F11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84879-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84879-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84879-5-RR against Human Methylmalonyl Coenzyme A mutase/MUT were performed. The affinity constant is 1.83 nM.

Methylmalonyl Coenzyme A mutase/MUT {ptg:Brand} Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Cat No. 84879-5-PBS

Clone No.242198F11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

MUT, Methylmalonyl-CoA isomerase, Methylmalonyl Coenzyme A mutase, Methylmalonyl CoA isomerase, MCM

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Product Information

84879-5-PBS targets Methylmalonyl Coenzyme A mutase/MUT in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Methylmalonyl Coenzyme A mutase/MUT fusion protein Ag10523
• Full Name: methylmalonyl Coenzyme A mutase
• Calculated Molecular Weight: 750 aa, 83 kDa
• Observed Molecular Weight: 78 kDa
• GenBank Accession Number: BC016282
• Gene Symbol: MUT
• Gene ID (NCBI): 4594
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P22033
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Methylmalonyl Coenzyme A mutase (MUT) is an enzyme that plays a crucial role in the metabolism of certain amino acids and fatty acids. Mutations in the MUT gene can lead to methylmalonic acidemia, a metabolic disorder characterized by the accumulation of toxic compounds such as methylmalonyl-CoA and propionyl-CoA. This condition can cause severe health issues including developmental delays, metabolic acidosis, and neurological problems. MUT is essential for maintaining normal metabolic processes and its dysfunction can have significant health implications, highlighting its importance in both basic metabolism and clinical medicine.