Mesothelin Monoclonal antibody, PBS Only

Mesothelin Monoclonal Antibody for Indirect ELISA, IHC, WB

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Indirect ELISA, IHC, WB

Conjugate

Unconjugated

CloneNo.

2E3A4

Cat no : 66404-1-PBS

Synonyms

MSLN, MPF Mesothelin, cleaved form, MPF, Mesothelin, cleaved form, Megakaryocyte-potentiating factor

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Validation Data Gallery

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  WB analysis of HeLa using 66404-1-Ig (same clone as 66404-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66404-1-Ig (Mesothelin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66404-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66404-1-Ig (same clone as 66404-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66404-1-Ig (Mesothelin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66404-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 66404-1-Ig (same clone as 66404-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66404-1-Ig (Mesothelin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66404-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 66404-1-Ig (same clone as 66404-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66404-1-Ig (Mesothelin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66404-1-PBS in a different storage buffer formulation.

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Product Information

66404-1-PBS targets Mesothelin in Indirect ELISA, IHC, WB applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Mesothelin fusion protein Ag23606
• Full Name: mesothelin
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 69 kDa
• GenBank Accession Number: BC003512
• Gene Symbol: MSLN
• Gene ID (NCBI): 10232
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Mesothelin (MSLN) is a 40kDa GPI-anchored surface glycoprotein that is expressed on pleural and peritoneal mesothelial cells, but not in other non-neoplastic tissues. The mesothelin gene encodes a 69-71kDa precursor protein that is processed to a 40kDa membrane-bound protein termed mesothelin and a 31kDa shed fragment called megakaryocyte-potentiating factor (MPF) that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues (e.g. mesothelium), and is expressed at high levels in mesotheliomas, as well as ovarian, pancreatic, and lung cancers. Mesothelin may be a new target for immunotherapy (PMID: 20922056, 18691948, 15217923).