Mouse MCP-1 Recombinant antibody, PBS Only

MCP-1 Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230483F11

Cat no : 83157-5-PBS

Synonyms

AI323594, Ccl2, chemokine (C C motif) ligand 2, HC11, JE, MCAF, MCP 1, MCP1, Scya2, Sigje, SMC CF

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Validation Data Gallery

• 

  WB analysis of RAW 264.7 using 83157-5-RR (same clone as 83157-5-PBS)

  Un-treated, LPS and Brefeldin A treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 83157-5-RR (Mcp1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83157-5-PBS in a different storage buffer formulation.

• 

  Affinity experiments of 83157-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83157-5-RR against Mouse MCP-1 were performed. The affinity constant is 60.5 pM.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83157-5-PBS is MCP-1 Fusion Protein expressed in E. coli.

• Tested Reactivity: Mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: chemokine (C-C motif) ligand 2
• GenBank Accession Number: NM-011333
• Gene Symbol: Ccl2
• Gene ID (NCBI): 20296
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.