Mouse MCP-1/CCL2 Recombinant antibody, PBS Only (Capture/Detector)

MCP-1/CCL2 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230483H3

Cat no : 83157-4-PBS

Synonyms

Ccl2, MCP1, MCP-1, chemokine (C C motif) ligand 2, CCL-2

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00168-4

  Sandwich ELISA standard curve of MP00168-4, Mouse MCP-1 Recombinant Matched Antibody Pair - PBS only. 83157-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0427. 83157-4-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

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  Sample test of MP00168-4

  J774A.1 mouse reticulum cell sarcoma macrophage cells (1 x 10*6 cells/mL) were cultured for 3 days in DMEM containing 10% fetal bovine serum. Cells were untreated or treated with 500 ng/mL recombinant mouse IFN-γ and 10 ng/mL LPS. An aliquot of the cell culture supernatants were removed, assayed for mouse MCP-1, and measured 6,956.6 pg/mL and 58,869.9 pg/mL.

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  Sample test of MP00168-4

  Serum of sixteen mice was measured. The MCP-1 concentration of detected samples was determined to be 77.0 pg/mL with a range of 39.7 - 135.3 pg/mL.

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  Cytometric bead array standard curve of MP00168-1

  Cytometric bead array standard curve of MP00168-1, Mouse Ccl2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83157-4-PBS. Detection antibody: 83157-2-PBS. Standard: Eg0427. Range: 0.625-20 ng/mL.

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  Cytometric bead array standard curve of MP00168-3

  Cytometric bead array standard curve of MP00168-3, Mouse Ccl2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83157-4-PBS. Detection antibody: 83157-1-PBS. Standard: Eg0427. Range: 1.25-20 ng/mL.

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Product Information

83157-4-PBS targets MCP-1/CCL2 as part of a matched antibody pair:

MP00168-1: 83157-4-PBS capture and 83157-2-PBS detection (validated in Cytometric bead array)

MP00168-3: 83157-4-PBS capture and 83157-1-PBS detection (validated in Cytometric bead array)

MP00168-4: 83157-6-PBS capture and 83157-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: chemokine (C-C motif) ligand 2
• GenBank Accession Number: NM-011333
• Gene Symbol: MCP-1/CCL2
• Gene ID (NCBI): 20296
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.