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MYOD1 Monoclonal antibody, PBS Only

MYOD1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

5D1D12

Cat no : 66214-1-PBS

Synonyms

bHLHc1, Myf 3, MYF3, MYOD, MYOD1, myogenic differentiation 1, Myogenic factor 3, PUM

Validation Data Gallery

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  • Western Blot (WB) analysis of human skeletal muscle tissue using MYOD1 Monoclonal antibody (66214-1-Ig)

    WB analysis of human skeletal muscle using 66214-1-Ig (same clone as 66214-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66214-1-Ig (MYOD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66214-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66214-1-PBS targets MYOD1 in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MYOD1 fusion protein Ag13512
Full Name myogenic differentiation 1
Calculated Molecular Weight 320 aa, 35 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC064493
Gene Symbol MYOD1
Gene ID (NCBI) 4654
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

MYOD1, also named as BHLHC1 or MYF3, is a 320 amino acid protein, which promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation. This phosphorylation promotes its function in muscle differentiation. MYOD1 acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. MYOD1 together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. he calculated molecular weight of MYOD1 is 34 kDa, but modified MYOD1 is about 45-47 kDa. (PMID: 12037670)