MYOD1 Monoclonal antibody
MYOD1 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
5D1D12
Cat no : 66214-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human skeletal muscle tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
Product Information
66214-1-Ig targets MYOD1 in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | MYOD1 fusion protein Ag13512 |
| Full Name | myogenic differentiation 1 |
| Calculated Molecular Weight | 320 aa, 35 kDa |
| Observed Molecular Weight | 47 kDa |
| GenBank Accession Number | BC064493 |
| Gene Symbol | MYOD1 |
| Gene ID (NCBI) | 4654 |
| RRID | AB_2881605 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MYOD1, also named as BHLHC1 or MYF3, is a 320 amino acid protein, which promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation. This phosphorylation promotes its function in muscle differentiation. MYOD1 acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. MYOD1 together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. he calculated molecular weight of MYOD1 is 34 kDa, but modified MYOD1 is about 45-47 kDa. (PMID: 12037670)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MYOD1 antibody 66214-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Aging (Albany NY) Vitellogenin 2 promotes muscle development and stimulates the browning of white fat | ||
Front Oncol Deoxypodophyllotoxin Inhibits Non-Small Cell Lung Cancer Cell Growth by Reducing HIF-1α-Mediated Glycolysis. | ||
Toxicol Appl Pharmacol A novel oleanolic acid derivative HA-19 ameliorates muscle atrophy via promoting protein synthesis and preventing protein degradation. | ||
Int J Stem Cells Synergistic Effect of Hydrogen and 5-Aza on Myogenic Differentiation through the p38 MAPK Signaling Pathway in Adipose-Derived Mesenchymal Stem Cells | ||
Anim Biotechnol Retinoic acid signalling inhibits myogenesis by blocking MYOD translation in pig skeletal muscle cells |
