MYLK3 Recombinant antibody

MYLK3 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240762G7

Cat no : 83673-3-RR

Synonyms

MLCK, EC:2.7.11.18, Cardiac-MyBP-C-associated Ca/CaM kinase, Cardiac-MLCK, caMLCK

---

Validation Data Gallery

• 

  WB analysis of L02 using 83673-3-RR

  L02 cells were subjected to SDS PAGE followed by western blot with 83673-3-RR (MYLK3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 83673-3-RR

  human heart tissue were subjected to SDS PAGE followed by western blot with 83673-3-RR (MYLK3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 83673-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83673-3-RR against Human MYLK3 were performed. The affinity constant is below 1 pM.

---

Tested Applications

• Positive WB detected in: L02 cells, human heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83673-3-RR is MYLK3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MYLK3 fusion protein Ag16137
• Full Name: myosin light chain kinase 3
• Calculated Molecular Weight: 819 aa, 88 kDa
• Observed Molecular Weight: 95 kDa
• GenBank Accession Number: BC109097
• Gene Symbol: MYLK3
• Gene ID (NCBI): 91807
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cardiac myosin light chain kinase (cMLCK) encoded by the myosin light chain kinase 3 (MYLK3) phosphorylates cardiac myosin light chain 2 (MLC2, encoded by MYL2) and facilitates actin-myosin interactions that enhance contractile force in the animal heart (PMID: 29235529).