MYH9 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

MYH9 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5D9D2

Cat no : 60233-1-PBS

Synonyms

NMMHC II-a, Myosin-9, Myosin heavy chain, non-muscle IIa, Myosin heavy chain 9, Myosin 9

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Validation Data Gallery

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  WB analysis of mouse kidney using 60233-1-Ig (same clone as 60233-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60233-1-Ig (same clone as 60233-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse colon using 60233-1-Ig (same clone as 60233-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human kidney using 60233-1-Ig (same clone as 60233-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig spleen using 60233-1-Ig (same clone as 60233-1-PBS)

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig pancreas using 60233-1-Ig (same clone as 60233-1-PBS)

  pig pancreas tissue were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 60233-1-Ig (same clone as 60233-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 60233-1-Ig (MYH9 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60233-1-Ig (same clone as 60233-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 60233-1-Ig (MYH9 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 60233-1-Ig (same clone as 60233-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using 60233-1-Ig (MYH9 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

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Product Information

60233-1-PBS targets MYH9 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, pig samples.

• Tested Reactivity: human, mouse, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MYH9 fusion protein Ag1612
• Full Name: myosin, heavy chain 9, non-muscle
• Calculated Molecular Weight: 227 kDa
• Observed Molecular Weight: 230 kDa, 160 kDa
• GenBank Accession Number: BC011915
• Gene Symbol: MYH9
• Gene ID (NCBI): 4627
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MYH9 (Myosin-9) appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. MYH9 is a hexameric protein that consists of two heavy chains (~200 kDa) and two pairs of non-identical light chains (15-22 kDa), a pair of essential or alkali light chains, and a pair of regulatory light chains (PMID: 12800156).