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MYH9 Monoclonal antibody
MYH9 Monoclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, pig
Applications
WB, IHC, IF/ICC, CoIP, ELISA
Conjugate
Unconjugated
CloneNo.
5D9D2
Cat no : 60233-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse kidney tissue, human kidney tissue, pig spleen tissue, U2OS cells, mouse colon tissue, pig pancreas tissue, A431 cells, human placenta tissue, pig kidney tissue |
Positive IF/ICC detected in | HepG2 cells, U2OS cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 2 publications below |
WB | See 6 publications below |
IHC | See 2 publications below |
IF | See 6 publications below |
CoIP | See 1 publications below |
Product Information
60233-1-Ig targets MYH9 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, pig samples.
Tested Reactivity | human, mouse, pig |
Cited Reactivity | human, mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | MYH9 fusion protein Ag1612 |
Full Name | myosin, heavy chain 9, non-muscle |
Calculated Molecular Weight | 227 kDa |
Observed Molecular Weight | 230 kDa, 160 kDa |
GenBank Accession Number | BC011915 |
Gene Symbol | MYH9 |
Gene ID (NCBI) | 4627 |
RRID | AB_2881357 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MYH9 (Myosin-9) appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. MYH9 is a hexameric protein that consists of two heavy chains (~200 kDa) and two pairs of non-identical light chains (15-22 kDa), a pair of essential or alkali light chains, and a pair of regulatory light chains (PMID: 12800156).
Protocols
Product Specific Protocols | |
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WB protocol for MYH9 antibody 60233-1-Ig | Download protocol |
IF protocol for MYH9 antibody 60233-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun Lymphocyte-specific protein 1 regulates mechanosensory oscillation of podosomes and actin isoform-based actomyosin symmetry breaking. | ||
Cell Death Differ Glycoprotein PTGDS promotes tumorigenesis of diffuse large B-cell lymphoma by MYH9-mediated regulation of Wnt-β-catenin-STAT3 signaling
| ||
Cell Rep Mechanotransduction in response to ECM stiffening impairs cGAS immune signaling in tumor cells | ||
Theranostics Salvia miltiorrhiza-derived miRNAs suppress vascular remodeling through regulating OTUD7B/KLF4/NMHC IIA axis. | ||
J Autoimmun Antibodies in cerebral cavernous malformations react with cytoskeleton autoantigens in the lesional milieu. |