MYB/c-Myb Recombinant antibody, PBS Only

• 

  WB analysis using 82804-2-RR (same clone as 82804-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82804-2-RR (MYB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82804-2-PBS in a different storage buffer formulation.

• 

  FC experiment of MCF-7 using 82804-2-RR (same clone as 82804-2-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug MYB/c-Myb Recombinant antibody (82804-2-RR, Clone:230179C11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82804-2-PBS in a different storage buffer formulation.

MYB/c-Myb {ptg:Brand} Recombinant Antibody for Indirect ELISA, WB

Cat No. 82804-2-PBS

Clone No.230179C11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Indirect ELISA, WB

Synonyms

MYB, c-Myb, Transcriptional activator Myb, Proto-oncogene c-Myb, Proto oncogene c Myb

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Product Information

82804-2-PBS targets MYB/c-Myb in Indirect ELISA, WB applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MYB/c-Myb fusion protein Ag12021
• Full Name: v-myb myeloblastosis viral oncogene homolog (avian)
• Calculated Molecular Weight: 640 aa, 72 kDa
• Observed Molecular Weight: 40-50 kDa, 72-80 kDa
• GenBank Accession Number: BC064955
• Gene Symbol: c-Myb
• Gene ID (NCBI): 4602
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P10242
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

The MYB protein is a transcription factor that plays a key role in regulating the proliferation/differentiation of progenitor cells in bone marrow, colonic crypts, and neurogenic niches [PMID:18574464]. It is involved in the regulation of epithelial-to-mesenchymal transition (EMT) and invasion in neuroblastoma, colon carcinoma, and embryonic kidney cells through the upregulation of the transcription repressor Slug [PMID:20622260]. It also a DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3', and plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. MYB exists some isoforms with MV 85, 75, 72, 68, 63 and 40 kDa.