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MYB/c-Myb Recombinant antibody

MYB/c-Myb Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230179C11

Cat no : 82804-2-RR

Synonyms

MYB, c-Myb, Transcriptional activator Myb, Proto-oncogene c-Myb, Proto oncogene c Myb

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYB/c-Myb Recombinant antibody (82804-2-RR)

    WB analysis using 82804-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82804-2-RR (MYB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of MCF-7 cells using MYB/c-Myb Recombinant antibody (82804-2-RR)

    FC experiment of MCF-7 using 82804-2-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug MYB/c-Myb Recombinant antibody (82804-2-RR, Clone:230179C11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, MOLT-4 cells, HT-29 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82804-2-RR targets MYB/c-Myb in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MYB/c-Myb fusion protein Ag12021
Full Name v-myb myeloblastosis viral oncogene homolog (avian)
Calculated Molecular Weight 640 aa, 72 kDa
Observed Molecular Weight40-50 kDa, 72-80 kDa
GenBank Accession NumberBC064955
Gene Symbol c-Myb
Gene ID (NCBI) 4602
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The MYB protein is a transcription factor that plays a key role in regulating the proliferation/differentiation of progenitor cells in bone marrow, colonic crypts, and neurogenic niches [PMID:18574464]. It is involved in the regulation of epithelial-to-mesenchymal transition (EMT) and invasion in neuroblastoma, colon carcinoma, and embryonic kidney cells through the upregulation of the transcription repressor Slug [PMID:20622260]. It also a DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3', and plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. MYB exists some isoforms with MV 85, 75, 72, 68, 63 and 40 kDa.

Protocols

Product Specific Protocols
WB protocol for MYB/c-Myb antibody 82804-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols