CoraLite® Plus 488-conjugated MX1 Recombinant antibody

MX1 Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2L19

Cat no : CL488-82677

Synonyms

IFI 78K, IFI78, Interferon induced protein p78, MX, MX1, MxA, Myxovirus resistance protein 1

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Validation Data Gallery

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  IF Staining of A431 using CL488-82677

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using CoraLite® Plus 488 MX1 antibody (CL488-82677, Clone: 2L19 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82677 targets MX1 in IF/ICC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MX1 fusion protein Ag4692
• Full Name: Myxovirus resistance protein 1
• Calculated Molecular Weight: 662 aa, 76 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC032602
• Gene Symbol: MX1
• Gene ID (NCBI): 4599
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Mx1 (also known as MxA) is a GTPase that belongs to the Mx family of proteins, which are ubiquitous in eukaryotes and involved in the immune response to viral infections. The human Mx1 is localized in the cytoplasm interfering with the translation of viral proteins, whereas the mouse Mx1 accumulates in the nucleus and inhibits the primary transcription of viral RNA. Mx1 expression is induced by treatment with α/β IFN.