MUM1 Monoclonal antibody, PBS Only

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  WB analysis of HEK-293 using 60353-1-Ig (same clone as 60353-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60353-1-Ig (MUM1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60353-1-PBS in a different storage buffer formulation.

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  WB analysis of A375 using 60353-1-Ig (same clone as 60353-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 60353-1-Ig (MUM1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60353-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 60353-1-Ig (same clone as 60353-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60353-1-Ig (MUM1 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60353-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60353-1-Ig (same clone as 60353-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MUM1 antibody (60353-1-Ig, Clone: 3B1D2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60353-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A375 using 60353-1-Ig (same clone as 60353-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using 60353-1-Ig (MUM1 antibody), at dilution of 1:50 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60353-1-PBS in a different storage buffer formulation.

MUM1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 60353-1-PBS

Clone No.3B1D2

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

EXPAND1, HSPC211, MUM 1, MUM1, Protein expandere

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Product Information

60353-1-PBS targets MUM1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: MUM1 fusion protein Ag3371
• Full Name: melanoma associated antigen (mutated) 1
• Calculated Molecular Weight: 18 kDa, 79 kDa
• Observed Molecular Weight: 78-80 kDa
• GenBank Accession Number: BC008098
• Gene Symbol: MUM1
• Gene ID (NCBI): 84939
• RRID: AB_2881462
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q2TAK8
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

MUM1, also named as EXPAND1, is an architectural component of the chromatin, which in response to DNA damage serves as an accessory factor to promote cell survival. It is a 53BP1-associated DNA damage-responsive factor. MUM1/EXPAND1 is different to another protein IRF4/MUM1.