MUM1 Monoclonal antibody

MUM1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

3B1D2

Cat no : 60353-1-Ig

Synonyms

EXPAND1, HSPC211, MUM 1, MUM1, Protein expandere

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Validation Data Gallery

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  WB analysis of HEK-293 using 60353-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60353-1-Ig (MUM1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 60353-1-Ig

  A375 cells were subjected to SDS PAGE followed by western blot with 60353-1-Ig (MUM1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 60353-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60353-1-Ig (MUM1 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 60353-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MUM1 antibody (60353-1-Ig, Clone: 3B1D2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of A375 using 60353-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using 60353-1-Ig (MUM1 antibody), at dilution of 1:50 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, A375 cells, Jurkat cells
• Positive IF/ICC detected in: HeLa cells, A375 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

60353-1-Ig targets MUM1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: MUM1 fusion protein Ag3371
• Full Name: melanoma associated antigen (mutated) 1
• Calculated Molecular Weight: 18 kDa, 79 kDa
• Observed Molecular Weight: 78-80 kDa
• GenBank Accession Number: BC008098
• Gene Symbol: MUM1
• Gene ID (NCBI): 84939
• RRID: AB_2881462
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MUM1, also named as EXPAND1, is an architectural component of the chromatin, which in response to DNA damage serves as an accessory factor to promote cell survival. It is a 53BP1-associated DNA damage-responsive factor. MUM1/EXPAND1 is different to another protein IRF4/MUM1.

Publications

Species	Application	Title
human	IHC	Front Oncol; Clinical Impact of the Histopathological Index and Neuroimaging Features Status in Primary Central Nervous System Diffuse Large B-Cell Lymphoma: A Single-Center Retrospective Analysis of 51 Cases.; Authors - Zhou Qi