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MUC20 Polyclonal antibody, PBS Only

MUC20 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 30457-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MUC20 Polyclonal antibody (30457-1-AP)

    WB analysis using 30457-1-AP (same clone as 30457-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30457-1-AP (MUC20 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30457-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MUC20 Polyclonal antibody (30457-1-AP)

    IF Staining of HepG2 using 30457-1-AP (same clone as 30457-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MUC20 antibody (30457-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30457-1-PBS in a different storage buffer formulation.

Product Information

30457-1-PBS targets MUC20 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MUC20 fusion protein Ag31867
Full Name mucin 20, cell surface associated
Calculated Molecular Weight 55 kDa, 71 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC029267
Gene Symbol MUC20
Gene ID (NCBI) 200958
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MUC20 contains serine, threonine, and proline-rich repeats in its extracellular domain and is likely to be extensively O-glycosylated. MUC20 blocks GRB2 recruitment to MET thus suppressing the GRB2-RAS pathway and inhibiting HGF-induced proliferation of MMP1 and MMP9 expression (PMID: 15314156). MUC20 is highly expressed in the kidney and localized in the proximal tubules but not in the glomerulus or distal tubules(PMID: 14565953). MUC20 was detected in most of the male urogenital tract epithelia, except for epididymis(PMID: 16997931). The expression of MUC20 is up-regulated in the kidneys of patients with immunoglobulin A nephropathy, in an animal model of lupus nephritis, and in mice with acute renal injury caused by cisplatin administration or unilateral ureteral obstruction(PMID: 14565953).