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MUC20 Polyclonal antibody

MUC20 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30457-1-AP

Synonyms

FLJ14408, FLJ53153, KIAA1359, MUC 20, MUC20, Mucin 20

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MUC20 Polyclonal antibody (30457-1-AP)

    WB analysis using 30457-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30457-1-AP (MUC20 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MUC20 Polyclonal antibody (30457-1-AP)

    IF Staining of HepG2 using 30457-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MUC20 antibody (30457-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse kidney tissue, mouse liver tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30457-1-AP targets MUC20 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MUC20 fusion protein Ag31867
Full Name mucin 20, cell surface associated
Calculated Molecular Weight 55 kDa, 71 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC029267
Gene Symbol MUC20
Gene ID (NCBI) 200958
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MUC20 contains serine, threonine, and proline-rich repeats in its extracellular domain and is likely to be extensively O-glycosylated. MUC20 blocks GRB2 recruitment to MET thus suppressing the GRB2-RAS pathway and inhibiting HGF-induced proliferation of MMP1 and MMP9 expression (PMID: 15314156). MUC20 is highly expressed in the kidney and localized in the proximal tubules but not in the glomerulus or distal tubules(PMID: 14565953). MUC20 was detected in most of the male urogenital tract epithelia, except for epididymis(PMID: 16997931). The expression of MUC20 is up-regulated in the kidneys of patients with immunoglobulin A nephropathy, in an animal model of lupus nephritis, and in mice with acute renal injury caused by cisplatin administration or unilateral ureteral obstruction(PMID: 14565953).

Protocols

Product Specific Protocols
WB protocol for MUC20 antibody 30457-1-APDownload protocol
IF protocol for MUC20 antibody 30457-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols