MTMR15 Recombinant antibody

MTMR15 Uni-rAb^TM Recombinant Antibody for FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240629E4

Cat no : 83622-6-RR

Synonyms

hFAN1, Fanconi-associated nuclease 1, FANCD2/FANCI-associated nuclease 1, FAN1, EC:3.1.21.-

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Validation Data Gallery

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  FC experiment of K-562 using 83622-6-RR

  1x10^6 K-562 cells were intracellularly stained with 0.25 ug Mtmr15 Recombinant Antibody (83622-6-RR, Clone:240629E4) (red), or 0.25 ug Isotype Control (blue) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of A431 using 83622-6-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug Mtmr15 Recombinant Antibody (83622-6-RR, Clone:240629E4)(red), or 0.25 ug Isotype Control (blue) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  Affinity and Kinetic Characterization of 83622-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83622-6-RR against Human MTMR15 were performed. The affinity constant is 3.74 nM.

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Tested Applications

• Positive FC (Intra) detected in: K-562 cells, A431 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83622-6-RR is MTMR15 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MTMR15 fusion protein Ag11651
• Full Name: myotubularin related protein 15
• Calculated Molecular Weight: 60 kDa, 114 kDa
• GenBank Accession Number: BC047882
• Gene Symbol: MTMR15
• Gene ID (NCBI): 22909
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTMR15, also named as FAN1 and KIAA1018, is a nuclease required for the repair of DNA interstrand cross-links (ICL) recruited at sites of DNA damage by monoubiquitinated FANCD2. It is specifically involved in repair of ICL-induced DNA breaks by being required for efficient homologous recombination, probably in the resolution of homologous recombination intermediates. MTMR15 has some isoforms with MW 114 kDa and 60 kDa. The MW will be migrated to 120-140 kDa and 63-68 kDa with modification.