MTMR15 Recombinant antibody, PBS Only

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  FC experiment of K-562 using 83622-6-RR (same clone as 83622-6-PBS)

  1x10^6 K-562 cells were intracellularly stained with 0.25 ug Mtmr15 Recombinant Antibody (83622-6-RR, Clone:240629E4) (red), or 0.25 ug Isotype Control (blue) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83622-6-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 83622-6-RR (same clone as 83622-6-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug Mtmr15 Recombinant Antibody (83622-6-RR, Clone:240629E4)(red), or 0.25 ug Isotype Control (blue) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83622-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83622-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83622-6-RR against Human MTMR15 were performed. The affinity constant is 3.74 nM.

MTMR15 {ptg:Brand} Recombinant Antibody for FC (Intra), Indirect ELISA

Cat No. 83622-6-PBS

Clone No.240629E4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), Indirect ELISA

Synonyms

hFAN1, Fanconi-associated nuclease 1, FANCD2/FANCI-associated nuclease 1, FAN1, EC:3.1.21.-

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Product Information

83622-6-PBS targets MTMR15 in FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MTMR15 fusion protein Ag11651
• Full Name: myotubularin related protein 15
• Calculated Molecular Weight: 60 kDa, 114 kDa
• GenBank Accession Number: BC047882
• Gene Symbol: MTMR15
• Gene ID (NCBI): 22909
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9Y2M0
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

MTMR15, also named as FAN1 and KIAA1018, is a nuclease required for the repair of DNA interstrand cross-links (ICL) recruited at sites of DNA damage by monoubiquitinated FANCD2. It is specifically involved in repair of ICL-induced DNA breaks by being required for efficient homologous recombination, probably in the resolution of homologous recombination intermediates. MTMR15 has some isoforms with MW 114 kDa and 60 kDa. The MW will be migrated to 120-140 kDa and 63-68 kDa with modification.