MTHFD1L Monoclonal antibody, PBS Only (Capture)

MTHFD1L Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, rabbit

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G1F2

Cat no : 68321-1-PBS

Synonyms

MTC1THFS, Monofunctional C1-tetrahydrofolate synthase, mitochondrial, FTHFSDC1, EC:6.3.4.3, 1G1F2

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Validation Data Gallery

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  WB analysis using 68321-1-Ig (same clone as 68321-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68321-1-Ig (MTHFD1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 68321-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68321-1-Ig (same clone as 68321-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68321-1-Ig (MTHFD1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 68321-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 68321-1-Ig (same clone as 68321-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68321-1-Ig (MTHFD1L antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68321-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 68321-1-Ig (same clone as 68321-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68321-1-Ig (MTHFD1L antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68321-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 68321-1-Ig (same clone as 68321-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug MTHFD1L Monoclonal antibody (68321-1-Ig, Clone:1G1F2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68321-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50271-2

  Cytometric bead array standard curve of MP50271-2, MTHFD1L Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68321-1-PBS. Detection antibody: 68321-3-PBS. Standard:Ag9061. Range: 0.195-50 ng/mL.

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Product Information

68321-1-PBS targets MTHFD1L as part of a matched antibody pair:

MP50271-2: 68321-1-PBS capture and 68321-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat, rabbit
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: MTHFD1L fusion protein Ag9061
• Full Name: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like
• Calculated Molecular Weight: 978 aa, 106 kDa
• Observed Molecular Weight: 106 kDa
• GenBank Accession Number: BC017477
• Gene Symbol: MTHFD1L
• Gene ID (NCBI): 25902
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MTHFD1L(Monofunctional C1-tetrahydrofolate synthase, mitochondrial) is also named as FTHFSDC1(Formyltetrahydrofolate synthetase). MTHFD1L enzyme is present in mitochondria from normal embryonic tissues and embryonic fibroblast cell lines, and embryonic mitochondria possess the ability to synthesize formate from glycine. It catalyzes the final step in the mitochondrial conversion of 1-C units to formate in embryos. Moreover, MTHFD1L levels were substantially higher in embryonic mitochondria than in adult liver mitochondria and embryonic mitochondria exhibited greater formate production(PMID:19948730). It has 2 isoforms produced by alternative splicing.