CoraLite®555-conjugated MTHFD1 Monoclonal antibody

MTHFD1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF/ICC

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

2B1G3

Cat no : CL555-67670

Synonyms

MTHFC, MTHFD, MTHFD1

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Validation Data Gallery

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  IF Staining of HEK-293 using CL555-67670

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite®555 MTHFD1 antibody (CL555-67670, Clone: 2B1G3 ) at dilution of 1:200.

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  IF Staining of HEK-293 using CL555-67670

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite®555 MTHFD1 antibody (CL555-67670, Clone: 2B1G3 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-67670 targets MTHFD1 in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MTHFD1 fusion protein Ag24553
• Full Name: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase
• Calculated Molecular Weight: 101 kDa
• Observed Molecular Weight: 101 kDa
• GenBank Accession Number: BC009806
• Gene Symbol: MTHFD1
• Gene ID (NCBI): 4522
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MTHFD1 is a trifunctional enzyme involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism. MTHFD1 is associated with HCC and CRC progression. It has been reported that MTHFD-deficient patient fibroblasts exhibit enriched MTHFD1 in the nucleus which is critical for de novo dTMP synthesis.(PMID: 31133746, 30997850, 31421459, 25548164)