MTH1 Monoclonal antibody, PBS Only

MTH1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D7G4

Cat no : 67443-1-PBS

Synonyms

8 oxo dGTPase, MTH1, Nudix motif 1, NUDT1

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Validation Data Gallery

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  WB analysis using 67443-1-Ig (same clone as 67443-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67443-1-Ig (MTH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67443-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 67443-1-Ig (same clone as 67443-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67443-1-Ig (MTH1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67443-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 67443-1-Ig (same clone as 67443-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67443-1-Ig (MTH1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67443-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67443-1-Ig (same clone as 67443-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67443-1-Ig (MTH1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67443-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67443-1-Ig (same clone as 67443-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67443-1-Ig (MTH1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67443-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67443-1-PBS targets MTH1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MTH1 fusion protein Ag9802
• Full Name: nudix (nucleoside diphosphate linked moiety X)-type motif 1
• Calculated Molecular Weight: 179aa,20 kDa; 197aa,23 kDa
• Observed Molecular Weight: 19 kDa
• GenBank Accession Number: BC014618
• Gene Symbol: NUDT1
• Gene ID (NCBI): 4521
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MTH1, also known as NUDT1 or 8-Oxo-dGTPase, is an important repair enzyme that prevents oxidative stress-induced DNA damage through hydrolyzing mutagenic 8-oxo-dGTP into 8-oxo-dGMP. MTH1 is expressed in postmitotic neurons as well as in proliferative tissues, and it is localized both in the mitochondria and nucleus. MTH1 might be a useful marker of oxidative stress and its level increased upon oxidative stress. Increased expression of MTH1 were also found in various cancer tissues and neurodegenerative diseases.