MTF2 Monoclonal antibody

• 

  WB analysis using 68713-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68713-1-Ig (MTF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

MTF2 Monoclonal Antibody for WB, ELISA

Cat No. 68713-1-Ig

Clone No.2E5D5

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Rat, Rabbit, Mouse

Applications

WB, ELISA

Synonyms

dJ976O13.2, hPCl2, M96, MTF2, PCL2, Polycomb like protein 2, RP5 976O13.1

---

Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, Rabbit testis tissue, Mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68713-1-Ig targets MTF2 in WB, ELISA applications and shows reactivity with Human, Rat, Rabbit, Mouse samples.

• Tested Reactivity: Human, Rat, Rabbit, Mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: MTF2 fusion protein Ag9384
• Full Name: metal response element binding transcription factor 2
• Calculated Molecular Weight: 536 aa, 61 kDa
• Observed Molecular Weight: 55 kDa, 60 kDa, 75 kDa
• GenBank Accession Number: BC010013
• Gene Symbol: MTF2
• Gene ID (NCBI): 22823
• RRID: AB_3670406
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y483
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTF2, also named as Metal-response element-binding transcription factor 2, is a 593 amino acid protein. It exists several isoforms and the range of the molecular weight of isoforms are about 55-60 kDa and 29 kDa. MTF2 contains 2 PHD-type zinc fingers and belongs to the Polycomblike family. MTF2 acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex, leading to enhance PRC2 H3K27me3 methylation activity and regulates the transcriptional networks during embryonic stem cell self-renewal and differentiation.