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CoraLite® Plus 488-conjugated MTAP Recombinant antibody

MTAP Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240030E5

Cat no : CL488-83005-5

Synonyms

MTA phosphorylase, MSAP, EC:2.4.2.28, c86fus, 5'-methylthioadenosine phosphorylase

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated MTAP Recombinant ant (CL488-83005-5)

    IF Staining of HeLa using CL488-83005-5

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 MTAP antibody (CL488-83005-5, Clone: 240030E5 ) at dilution of 1:200.

Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-83005-5 is MTAP Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MTAP fusion protein Ag2051
Full Name methylthioadenosine phosphorylase
Calculated Molecular Weight 283 aa, 31 kDa
GenBank Accession NumberBC018625
Gene Symbol MTAP
Gene ID (NCBI) 4507
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

MTAP is a 5-deoxy-5-methylthioadenosine (MTA) phosphorylase, converting MTA to salvageable intermediates including adenine and 5-methylthioribose-1-phosphate. MTAP is abundant in normal cells, but it is frequently found to be deleted in a variety of cancers. Its deficiency is common in cancer cell lines as well as in primary leukemia, lung cancer, melanoma, bladder cancer, gliomas and breast cancer.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 MTAP antibody CL488-83005-5Download protocol
Standard Protocols
Click here to view our Standard Protocols