MSH6 Monoclonal antibody

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MSH6 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

8

CloneNo.

2E10B2

Cat no : 66172-1-Ig

Synonyms

hMSH6, GTMBP, GTBP, G/T mismatch-binding protein, G/T mismatch binding protein

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Validation Data Gallery

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  WB analysis of HEK-293 using 66172-1-Ig

  WB result of MSH6 antibody (66172-1-Ig, 1:1000) with si-Control and si-MSH6 transfected HEK293 cells.

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  WB analysis of HEK-293 using 66172-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66172-1-Ig (MSH6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 66172-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 66172-1-Ig (MSH6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66172-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66172-1-Ig (MSH6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66172-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66172-1-Ig (MSH6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 66172-1-Ig

  IP result of anti-MSH6 (IP:66172-1-Ig, 4ug; Detection:66172-1-Ig 1:600) with HEK-293 cells lysate 1800ug.

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  IHC staining of human colon cancer using 66172-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66172-1-Ig (MSH6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66172-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66172-1-Ig (MSH6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66172-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66172-1-Ig (MSH6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66172-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66172-1-Ig (MSH6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66172-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66172-1-Ig (MSH6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66172-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66172-1-Ig (MSH6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U2OS using 66172-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MSH6 antibody (66172-1-Ig, Clone: 2E10B2 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

• 

  FC experiment of HEK-293 using 66172-1-Ig

  1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug MSH6 Monoclonal antibody (66172-1-Ig, Clone:2E10B2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, U2OS cells, A431 cells, HeLa cells, MCF-7 cells, K-562 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells
• Positive FC (Intra) detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 6 publications below

Product Information

66172-1-Ig targets MSH6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: MSH6 fusion protein Ag12645
• Full Name: mutS homolog 6 (E. coli)
• Calculated Molecular Weight: 153 kDa
• Observed Molecular Weight: 160 kDa
• GenBank Accession Number: BC004246
• Gene Symbol: MSH6
• Gene ID (NCBI): 2956
• RRID: AB_2881567
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MSH6, also named as DNA mismatch repair protein Msh6 or GTMBP, is a 1360 amino acid protein, which contains one PWWP domain and belongs to the DNA mismatch repair MutS family. MSH6 localizes in the nucleus and is a component of the post-replicative DNA mismatch repair system. Msh2 and Msh6 form a protein complex required to repair mismatches generated during DNA replication.

Publications

Species	Application	Title
mouse	WB	J Med Chem; CJ2: A Novel Potent Platinum(IV) Prodrug Enhances Chemo-Immunotherapy by Facilitating PD-L1 Degradation in the Cytoplasm and Cytomembrane; Authors - Renming Fan
human	WB	Respir Res; TMPRSS2 is a tumor suppressor and its downregulation promotes antitumor immunity and immunotherapy response in lung adenocarcinoma; Authors - Zhixian Liu
human	WB,IHC	Mol Carcinog; The mechanism of RGS5 regulating gastric cancer mismatch repair protein; Authors - Zhenwei Yang
human	IHC	Surgery; Mismatch repair status as a beneficial predictor of fluorouracil-based adjuvant chemotherapy for pancreatic cancer.; Authors - Dingkong Liang
human	IHC	J Surg Oncol; Clinicopathological and survival characteristic of mismatch repair status in ovarian clear cell carcinoma.; Authors - Jun Zhu
human	IHC	Neuropathology; Sebaceous adenoma occurring within an intracranial dermoid cyst.; Authors - Takashi Minamisaka