WB analysis using 82845-3-RR (same clone as 82845-3-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 82845-3-RR (MSH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82845-3-PBS in a different storage buffer formulation.
Various lysates were subjected to SDS PAGE followed by western blot with 82845-3-RR (MSH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82845-3-PBS in a different storage buffer formulation.
IF Staining of U-251 using 82845-3-RR (same clone as 82845-3-PBS)
Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MSH3 antibody (82845-3-RR, Clone: 1J23 ) at dilution of 1:1500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82845-3-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MSH3 antibody (82845-3-RR, Clone: 1J23 ) at dilution of 1:1500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82845-3-PBS in a different storage buffer formulation.
IF Staining of HeLa using 82845-3-PBS
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MSH3 antibody (82845-3-PBS, Clone: 1J23 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MSH3 antibody (82845-3-PBS, Clone: 1J23 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
IF Staining of U2OS using 82845-3-RR (same clone as 82845-3-PBS)
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MSH3 antibody (82845-3-RR, Clone: 1J23 ) at dilution of 1:1500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 82845-3-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MSH3 antibody (82845-3-RR, Clone: 1J23 ) at dilution of 1:1500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 82845-3-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 82845-3-RR
Biolayer interferometry (BLl) kinetic assays of 82845-3-RR against Human MSH3 were performed. The affinity constant is below 1 pM.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
MSH3 is a DNA mismatch repair (MMR) gene that heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. It undergoes frequent somatic mutation in colorectal cancers (CRCs) with MMR deficiency. MSH3 mismatch repair protein regulates sensitivity to cytotoxic drugs and a histone deacetylase inhibitor in human colon carcinoma cells (PMID: 23724141, PMID:32671096).
WB analysis using 82845-3-RR (same clone as 82845-3-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 82845-3-RR (MSH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82845-3-PBS in a different storage buffer formulation.
IF/ICC Figures
IF Staining of U-251 using 82845-3-RR (same clone as 82845-3-PBS)
Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MSH3 antibody (82845-3-RR, Clone: 1J23 ) at dilution of 1:1500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82845-3-PBS in a different storage buffer formulation.
IF Staining of HeLa using 82845-3-PBS
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MSH3 antibody (82845-3-PBS, Clone: 1J23 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
IF Staining of U2OS using 82845-3-RR (same clone as 82845-3-PBS)
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MSH3 antibody (82845-3-RR, Clone: 1J23 ) at dilution of 1:1500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 82845-3-PBS in a different storage buffer formulation.
AFFINITY Figures
Affinity and Kinetic Characterization of 82845-3-RR
Biolayer interferometry (BLl) kinetic assays of 82845-3-RR against Human MSH3 were performed. The affinity constant is below 1 pM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
MSH3 Recombinant antibody
Catalog Number
82845-3-PBS
Citations
-
Dilutions
IF/ICC : 1:125-1:500
Applications
WB, IF/ICC, Indirect ELISA
Reactivity
human, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
