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MSH3 Recombinant antibody

MSH3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1J23

Cat no : 82845-3-RR

Synonyms

Mismatch repair protein 1, hMSH3, DUG, DUC1, Divergent upstream protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MSH3 Recombinant antibody (82845-3-RR)

    WB analysis using 82845-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82845-3-RR (MSH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using MSH3 Recombinant antibody (82845-3-RR)

    IF Staining of U2OS using 82845-3-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MSH3 antibody (82845-3-RR, Clone: 1J23 ) at dilution of 1:1500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using MSH3 Recombinant antibody (82845-3-RR)

    IF Staining of U-251 using 82845-3-RR

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MSH3 antibody (82845-3-RR, Clone: 1J23 ) at dilution of 1:1500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Affinity and Kinetic Characterization of 82845-3-RR

    Affinity and Kinetic Characterization of 82845-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82845-3-RR against Human MSH3 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHSC-T6 cells
Positive IF/ICC detected inU-251 cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:750-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82845-3-RR targets MSH3 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MSH3 fusion protein Ag18060
Full Name mutS homolog 3 (E. coli)
Calculated Molecular Weight 1137 aa, 127 kDa
Observed Molecular Weight 127 kDa
GenBank Accession NumberBC130434
Gene Symbol MSH3
Gene ID (NCBI) 4437
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MSH3 is a DNA mismatch repair (MMR) gene that heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. It undergoes frequent somatic mutation in colorectal cancers (CRCs) with MMR deficiency. MSH3 mismatch repair protein regulates sensitivity to cytotoxic drugs and a histone deacetylase inhibitor in human colon carcinoma cells (PMID: 23724141, PMID:32671096).

Protocols

Product Specific Protocols
WB protocol for MSH3 antibody 82845-3-RRDownload protocol
IF protocol for MSH3 antibody 82845-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols