MSH2 Polyclonal antibody

• Featured Product
• KD/KO Validated

MSH2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

28

Cat no : 15520-1-AP

Synonyms

MSH 2

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Validation Data Gallery

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  WB analysis of HeLa using 15520-1-AP

  WB results of MSH2 (15520-1-AP, 1:5000) antibody with si-Control and si-MSH2 transfected HeLa cells..

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  WB analysis using 15520-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15520-1-AP (MSH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15520-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15520-1-AP (MSH2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15520-1-AP

  IP result of anti-MSH2 (IP:15520-1-AP, 5ug; Detection:15520-1-AP 1:500) with HeLa cells lysate 2000ug.

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  IHC staining of human stomach cancer using 15520-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15520-1-AP (MSH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 15520-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15520-1-AP (MSH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsil using 15520-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsil using 15520-1-AP (MSH2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human tonsil using 15520-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsil using 15520-1-AP (MSH2 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 15520-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MSH2 antibody (15520-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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  FC experiment of HeLa using 15520-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human MSH2 (15520-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: HeLa cells, Caco-2 cells, SW480 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human stomach cancer tissue, human tonsil tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 17 publications below
• IHC: See 13 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Product Information

15520-1-AP targets MSH2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MSH2 fusion protein Ag7835
• Full Name: mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
• Calculated Molecular Weight: 105 kDa
• Observed Molecular Weight: 100 kDa-105 kDa
• GenBank Accession Number: BC021566
• Gene Symbol: MSH2
• Gene ID (NCBI): 4436
• RRID: AB_2144799
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MSH2, named for its homologous to the E.coli MutS gene, takes part in DNA mismatch repair(MMR) to maintain the stability of DNA. MSH2 contains a helix-turn-helix domain, which responds for binding to DNA. Also, MSH2 has a direct role in mutation avoidance and micro-satellite stability in cell.

Publications

Species	Application	Title
human	WB	Leukemia; Targeting DNA polymerase β elicits synthetic lethality with mismatch repair deficiency in acute lymphoblastic leukemia; Authors - Ji-Yuan Teng ; KO Validated
human	WB	J Immunother Cancer; KALRN mutations promote antitumor immunity and immunotherapy response in cancer.; Authors - Mengyuan Li
human	WB, IHC	Hum Mol Genet; Lack of association of somatic CAG repeat expansion with striatal neurodegeneration in HD knock-in animal models.; Authors - Dazhang Bai ; KD Validated
human	WB	Cancer Prev Res (Phila); Estrogen stimulates the expression of mismatch repair gene hMLH1 in colonic epithelial cells.; Authors - Jin Peng P
human	IHC	Front Oncol; Molecular Classification in Patients With Endometrial Cancer After Fertility-Preserving Treatment: Application of ProMisE Classifier and Combination of Prognostic Evidence.; Authors - Xuting Ran
human	WB	J Biol Chem; Ectopic expression of human MutS homologue 2 on renal carcinoma cells is induced by oxidative stress with interleukin-18 promotion via p38 mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase (JNK) signaling pathways.; Authors - Mo Chen C