MRPS18A Recombinant antibody

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  WB analysis using 84201-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84201-1-RR (MRPS18A antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 84201-1-RR

  WB result of MRPS18A antibody (84201-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPS18A transfected HepG2 cells.

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  Affinity and Kinetic Characterization of 84201-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84201-1-RR against Human MRPS18A were performed. The affinity constant is below 1 pM.

• Featured Product
• KD/KO Validated

MRPS18A Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 84201-1-RR

Clone No.240850C3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

MRP S18 3, Large ribosomal subunit protein bS18a, HumanS18b, 39S ribosomal protein S18a, mitochondrial, 39S ribosomal protein S18-3, mitochondrial

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Tested Applications

• Positive WB detected in: HepG2 cells, A549 cells, HeLa cells, L02 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84201-1-RR targets MRPS18A in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MRPS18A fusion protein Ag9011
• Full Name: mitochondrial ribosomal protein S18A
• Calculated Molecular Weight: 196 aa, 22 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC010364
• Gene Symbol: MRPS18A
• Gene ID (NCBI): 55168
• RRID: AB_3671757
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9NVS2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MRPS18A (Mitochondrial Ribosomal Protein S18A) is a protein coding gene. It encodes a 28S subunit protein that belongs to the ribosomal protein S18P family. The increased expression of MRPS18A in cancer cells is explained by the increased energy metabolism of cancer cells and may pave the way for new diagnostic and therapeutic avenues to be explored (PMID: 28056857).