CoraLite® Plus 488-conjugated MRPS17 Polyclonal antibody

MRPS17 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-18881

Synonyms

S17mt, RPMS17, MRP-S17, MRPS 17, MRP S17

---

Validation Data Gallery

• 

  IF Staining of HepG2 using CL488-18881

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 MRPS17 antibody (CL488-18881) at dilution of 1:200, CL594-Phalloidin (red).

• 

  FC experiment of A549 using CL488-18881

  1x10^6 A549 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Mrps17 Polyclonal Antibody (CL488-18881)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: A549 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-18881 targets MRPS17 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MRPS17 fusion protein Ag13414
• Full Name: mitochondrial ribosomal protein S17
• Calculated Molecular Weight: 130 aa, 15 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC054031
• Gene Symbol: MRPS17
• Gene ID (NCBI): 51373
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.