MRPL38 Monoclonal antibody, PBS Only (Capture)

MRPL38 Monoclonal Antibody for WB, IP, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IP, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D11A1

Cat no : 68418-1-PBS

Synonyms

MRP L3, Large ribosomal subunit protein mL38, L38mt, KIAA1863, HSPC262

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Validation Data Gallery

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  WB analysis using 68418-1-Ig (same clone as 68418-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68418-1-Ig (MRPL38 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68418-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig liver using 68418-1-Ig (same clone as 68418-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 68418-1-Ig (MRPL38 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68418-1-PBS in a different storage buffer formulation.

• 

  IP experiment of A549 using 68418-1-Ig (same clone as 68418-1-PBS)

  IP result of anti-MRPL38 (IP:68418-1-Ig, 4ug; Detection:68418-1-Ig 1:2000) with A549 cells lysate 920 ug. This data was developed using the same antibody clone with 68418-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50538-3

  Cytometric bead array standard curve of MP50538-3, MRPL38 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68418-1-PBS. Detection antibody: 68418-2-PBS. Standard:Ag8927. Range: 1.563-100 ng/mL.

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  Cytometric bead array standard curve of MP50538-4

  Cytometric bead array standard curve of MP50538-4, MRPL38 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68418-1-PBS. Detection antibody: 68418-3-PBS. Standard:Ag8927. Range: 6.25-100 ng/mL.

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Product Information

68418-1-PBS targets MRPL38 as part of a matched antibody pair:

MP50538-3: 68418-1-PBS capture and 68418-2-PBS detection (validated in Cytometric bead array)

MP50538-4: 68418-1-PBS capture and 68418-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MRPL38 fusion protein Ag8927
• Full Name: mitochondrial ribosomal protein L38
• Calculated Molecular Weight: 346 aa, 41 kDa
• Observed Molecular Weight: 37-45 kDa
• GenBank Accession Number: BC013311
• Gene Symbol: MRPL38
• Gene ID (NCBI): 64978
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.