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MRPL38 Monoclonal antibody

MRPL38 Monoclonal Antibody for WB, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1D11A1

Cat no : 68418-1-Ig

Synonyms

FLJ13996, HSPC262, KIAA1863, L38mt, MRP L3, MRP L38, MRPL38, RPML3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MRPL38 Monoclonal antibody (68418-1-Ig)

    WB analysis using 68418-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68418-1-Ig (MRPL38 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of pig liver tissue using MRPL38 Monoclonal antibody (68418-1-Ig)

    WB analysis of pig liver using 68418-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 68418-1-Ig (MRPL38 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A549 cells using MRPL38 Monoclonal antibody (68418-1-Ig)

    IP experiment of A549 using 68418-1-Ig

    IP result of anti-MRPL38 (IP:68418-1-Ig, 4ug; Detection:68418-1-Ig 1:2000) with A549 cells lysate 920 ug.

Tested Applications

Positive WB detected inA549 cells, pig liver tissue, LNCaP cells, Jurkat cells, K-562 cells
Positive IP detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68418-1-Ig targets MRPL38 in WB, IP, ELISA applications and shows reactivity with Human, pig samples.

Tested Reactivity Human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MRPL38 fusion protein Ag8927
Full Name mitochondrial ribosomal protein L38
Calculated Molecular Weight 346 aa, 41 kDa
Observed Molecular Weight37-45 kDa
GenBank Accession NumberBC013311
Gene Symbol MRPL38
Gene ID (NCBI) 64978
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MRPL38 antibody 68418-1-IgDownload protocol
IP protocol for MRPL38 antibody 68418-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols