MRP1 Monoclonal antibody, PBS Only

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  WB analysis using 67228-1-Ig (same clone as 67228-1-PBS)

  Non-boil and boiled HepG2 and A549 cell lysates were subjected to SDS PAGE followed by western blot with 67228-1-Ig (ABCC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. Left panel was exposed for 20 sec. and right panel was exposed for 1 min. This data was developed using the same antibody clone with 67228-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 67228-1-Ig (same clone as 67228-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using MRP1 antibody (67228-1-Ig, Clone: 1G4A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67228-1-PBS in a different storage buffer formulation.

MRP1 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 67228-1-PBS

Clone No.1G4A2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

ABCC1, MRP1/ABCC1, 1G4A2

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Product Information

67228-1-PBS targets MRP1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MRP1 fusion protein Ag27048
• Full Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 1
• Calculated Molecular Weight: 172 kDa
• Observed Molecular Weight: 180-190 kDa
• GenBank Accession Number: NM_004996
• Gene Symbol: MRP1
• Gene ID (NCBI): 4363
• RRID: AB_2882516
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P33527
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Multidrug resistant-associated protein 1 (MRP1; ABCC1) is a member of the ATP-binding cassette (ABC) transporter protein superfamily, subfamily C. MRP1 is overexpressed in cancers and contributes to the occurrence of multidrug resistance (MDR). Expression of MRP1 has been considered as a negative marker for the outcome of chemotherapy. Recently MRP1 has been reported to play a crucial role in Aβ clearance at the blood-brain barrier.