MPO Recombinant antibody, PBS Only

• Featured Product
• KD/KO Validated

MPO Recombinant Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3J17

Cat no : 81610-1-PBS

Synonyms

MPO, myeloperoxidase

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Validation Data Gallery

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  WB analysis of HL-60 using 81610-1-RR (same clone as 81610-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 81610-1-RR (MPO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81610-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human saliva using 81610-1-RR (same clone as 81610-1-PBS)

  human saliva were subjected to SDS PAGE followed by western blot with 81610-1-RR (MPO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81610-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HL-60 using 81610-1-RR (same clone as 81610-1-PBS)

  WB result of MPO antibody (81610-1-RR; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MPO transfected HL-60 cells. This data was developed using the same antibody clone with 81610-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 81610-1-RR (same clone as 81610-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 81610-1-RR (MPO antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81610-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 81610-1-RR (same clone as 81610-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 81610-1-RR (MPO antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81610-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HL-60 using 81610-1-RR (same clone as 81610-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HL-60 cells using MPO antibody (81610-1-RR, Clone: 3J17 ) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81610-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81610-1-PBS targets MPO in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MPO fusion protein Ag17564
• Full Name: myeloperoxidase
• Calculated Molecular Weight: 745 aa, 84 kDa
• Observed Molecular Weight: 59 kDa, 90 kDa
• GenBank Accession Number: BC130476
• Gene Symbol: MPO
• Gene ID (NCBI): 4353
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). Fragments with molecular masses of 43-47 kDa were formed by autocatalysis during warming in sample buffer (PMID:12960244).The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing.