MOSC2 Recombinant antibody

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  WB analysis using 83705-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83705-2-RR (MOSC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 83705-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83705-2-RR (MOSC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 83705-2-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83705-2-RR (MOSC2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 83705-2-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83705-2-RR (MOSC2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HeLa using 83705-2-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MOSC2 Recombinant antibody (83705-2-RR, Clone:240747G6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83705-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83705-2-RR against Human MOSC2 were performed. The affinity constant is 0.473 nM.

MOSC2 {ptg:Brand} Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 83705-2-RR

Clone No.240747G6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

Molybdenum cofactor sulfurase C-terminal domain-containing protein 2, Moco sulfurase C-terminal domain-containing protein 2, Mitochondrial amidoxime reducing component 2, mARC2, EC:1.7.-.-

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Tested Applications

• Positive WB detected in: mouse liver tissue, HUVEC cells, HEK-293 cells, mouse kidney tissue, rat liver tissue
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83705-2-RR targets MOSC2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MOSC2 fusion protein Ag20694
• Full Name: MOCO sulphurase C-terminal domain containing 2
• Calculated Molecular Weight: 335 aa, 38 kDa
• Observed Molecular Weight: 35-38 kDa
• GenBank Accession Number: BC011973
• Gene Symbol: MOSC2
• Gene ID (NCBI): 54996
• RRID: AB_3671307
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q969Z3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MOSC domain-containing protein 2 (also known as MOSC2), also known as MARC2, is a component of prodrug-converting system, reduces a multitude of N-hydroxylated prodrugs particularly amidoximes, leading to increased drug bioavailability. Also, MOSC2 may be involved in mitochondrial N(omega)-hydroxy-L-arginine (NOHA) reduction, regulating endogenous nitric oxide levels and biosynthesis. The reductase activity is regulated under adipogenic conditions, and down-regulation of the terminal component MOSC2 resulted in decreased lipid synthesis, suggesting a possible physiological role of this enzyme system and its component MOSC2 in lipogenesis(PMID: 22203676).