MNT Recombinant antibody, PBS Only (Capture)

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  WB analysis using 83648-1-RR (same clone as 83648-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83648-1-RR (MNT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83648-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00617-1

  Sandwich ELISA standard curve of MP00617-1, Human MNT Recombinant Matched Antibody Pair - PBS only. 83648-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag34529. 83648-3-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

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  Sample test of MP00617-1

  The mean MNT concentration was determined to be 19.62 ng/mL in HEK-293T cell extract based on a 5.50 mg/mL extract load and 17.06 ng/mL in HeLa cell extract based on a 3.60 mg/mL extract load.

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  IF Staining of HeLa using 83648-1-RR (same clone as 83648-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MNT antibody (83648-1-RR, Clone: 240550A10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83648-1-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 83648-1-RR (same clone as 83648-1-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug MNT Recombinant antibody (83648-1-RR, Clone:240550A10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83648-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00617-1

  Cytometric bead array standard curve of MP00617-1, MNT Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83648-1-PBS. Detection antibody: 83648-3-PBS. Standard: Ag34529. Range: 0.625-80 ng/mL.

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  Cytometric bead array standard curve of MP00617-2

  Cytometric bead array standard curve of MP00617-2, MNT Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83648-1-PBS. Detection antibody: 83648-4-PBS. Standard: Ag34529. Range: 0.625-80 ng/mL.

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  Cytometric bead array standard curve of MP00617-3

  Cytometric bead array standard curve of MP00617-3, MNT Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83648-1-PBS. Detection antibody: 83648-2-PBS. Standard: Ag34529. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 83648-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83648-1-RR against Human MNT were performed. The affinity constant is below 1 pM.

MNT Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 83648-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Max binding protein MNT, MAX binding protein, MAD6, Class D basic helix-loop-helix protein 3, bHLHd3

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Product Information

83648-1-PBS targets MNT as part of a matched antibody pair:

MP00617-1: 83648-1-PBS capture and 83648-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00617-2: 83648-1-PBS capture and 83648-4-PBS detection (validated in Cytometric bead array)

MP00617-3: 83648-1-PBS capture and 83648-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MNT fusion protein Ag34529
• Full Name: MAX binding protein
• Calculated Molecular Weight: 582 aa, 62 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC117563
• Gene Symbol: MNT
• Gene ID (NCBI): 4335
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q99583
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Mnt (Max binding protein), also known as MAD6, ROX, bHLHd3 (class D basic helix-loop-helix protein 3) or MXD6, is a 582 amino acid nuclear protein that forms a complex with Max (Myc-associated factor X) to repress transcription. Mnt contains one basic helix-loop-helix (bHLH) domain and is encoded by a gene that maps to human chromosome 17p13.3. Chromosome 17 comprises over 2.5% of the human genome and encodes over 1,200 genes. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Tumor suppressor p53 is necessary for maintenance of cellular genetic integrity by moderating cell fate through DNA repair versus cell death. Like p53, BRCA1 is directly involved in DNA repair, though specifically it is recognized as a genetic determinant of early onset breast cancer and predisposition to cancers of the ovary, colon, prostate gland and fallopian tubes.