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MNT Recombinant antibody

MNT Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240550A10

Cat no : 83648-1-RR

Synonyms

Max binding protein MNT, MAX binding protein, MAD6, Class D basic helix-loop-helix protein 3, bHLHd3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MNT Recombinant antibody (83648-1-RR)

    WB analysis using 83648-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83648-1-RR (MNT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A431 cells using MNT Recombinant antibody (83648-1-RR)

    FC experiment of A431 using 83648-1-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug MNT Recombinant antibody (83648-1-RR, Clone:240550A10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83648-1-RR

    Affinity and Kinetic Characterization of 83648-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83648-1-RR against Human MNT were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, HEK-293T cells, Jurkat cells, A431 cells, HT-29 cells, C6 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83648-1-RR is MNT Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MNT fusion protein Ag34529
Full Name MAX binding protein
Calculated Molecular Weight 582 aa, 62 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC117563
Gene Symbol MNT
Gene ID (NCBI) 4335
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mnt (Max binding protein), also known as MAD6, ROX, bHLHd3 (class D basic helix-loop-helix protein 3) or MXD6, is a 582 amino acid nuclear protein that forms a complex with Max (Myc-associated factor X) to repress transcription. Mnt contains one basic helix-loop-helix (bHLH) domain and is encoded by a gene that maps to human chromosome 17p13.3. Chromosome 17 comprises over 2.5% of the human genome and encodes over 1,200 genes. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Tumor suppressor p53 is necessary for maintenance of cellular genetic integrity by moderating cell fate through DNA repair versus cell death. Like p53, BRCA1 is directly involved in DNA repair, though specifically it is recognized as a genetic determinant of early onset breast cancer and predisposition to cancers of the ovary, colon, prostate gland and fallopian tubes.

Protocols

Product Specific Protocols
WB protocol for MNT antibody 83648-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols