CoraLite®594-conjugated MMP9 (N-terminal) Polyclonal antibody

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• KD/KO Validated

MMP9 (N-terminal) Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Publications

1

Cat no : CL594-10375

Synonyms

MMP9, Matrix metalloproteinase 9, GELB, EC:3.4.24.35, CLG4B

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-10375

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 MMP9 (N-terminal) antibody (CL594-10375) at dilution of 1:200.

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  FC experiment of HeLa using CL594-10375

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human MMP9 (N-terminal) (CL594-10375) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL594-10375 targets MMP9 (N-terminal) in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MMP9 (N-terminal) fusion protein Ag0552
• Full Name: matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
• Calculated Molecular Weight: 707 aa, 78 kDa
• Observed Molecular Weight: 67 kDa, 92 kDa
• GenBank Accession Number: BC006093
• Gene Symbol: MMP9
• Gene ID (NCBI): 4318
• RRID: AB_2919732
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes, such as arthritis or metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. Matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) (MMP9, synonyms: GELB, CLG4B) degrades collagens type IV and V. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. The pro-MMP9 is 92 kDa, and it can be detected a processed form of 68 kDa. This protein can exist as a dimer of 180 kDa (PMID:7492685).

Publications

Species	Application	Title
human	IF	Cancers (Basel); Tumor-Associated Fibroblast-Derived Exosomal circDennd1b Promotes Pituitary Adenoma Progression by Modulating the miR-145-5p/ONECUT2 Axis and Activating the MAPK Pathway; Authors - Qian Jiang

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C