MMP7 Monoclonal antibody, PBS Only

MMP7 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E6D6

Cat no : 67990-1-PBS

Synonyms

Matrilysin, Matrin, Matrix metalloproteinase 7, MMP 7, MMP7, MPSL1, PUMP 1, Pump 1 protease, PUMP1, Uterine metalloproteinase

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Validation Data Gallery

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  WB analysis of SKOV-3 using 67990-1-Ig (same clone as 67990-1-PBS)

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 67990-1-Ig (MMP7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67990-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 67990-1-Ig (same clone as 67990-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 67990-1-Ig (MMP7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67990-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HT-29 using 67990-1-Ig (same clone as 67990-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67990-1-Ig (MMP7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67990-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 67990-1-Ig (same clone as 67990-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 67990-1-Ig (MMP7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67990-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 67990-1-Ig (same clone as 67990-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67990-1-Ig (MMP7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67990-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 67990-1-Ig (same clone as 67990-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67990-1-Ig (MMP7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67990-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human pancreas cancer using 67990-1-Ig (same clone as 67990-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using MMP7 antibody (67990-1-Ig, Clone: 2E6D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67990-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human pancreas cancer using 67990-1-Ig (same clone as 67990-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using MMP7 antibody (67990-1-Ig, Clone: 2E6D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67990-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human pancreas cancer using 67990-1-Ig (same clone as 67990-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using MMP7 antibody (67990-1-Ig, Clone: 2E6D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67990-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67990-1-PBS targets MMP7 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: MMP7 fusion protein Ag0550
• Full Name: matrix metallopeptidase 7 (matrilysin, uterine)
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 27-30 kDa
• GenBank Accession Number: BC003635
• Gene Symbol: MMP7
• Gene ID (NCBI): 4316
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.