MMP7 Monoclonal antibody, PBS Only (Detector)

MMP7 Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E6D6

Cat no : 67990-1-PBS

Synonyms

Matrix metalloproteinase-7, Matrix metalloproteinase 7, Matrin, Matrilysin, EC:3.4.24.23

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Validation Data Gallery

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  WB analysis of SKOV-3 using 67990-1-Ig (same clone as 67990-1-PBS)

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 67990-1-Ig (MMP7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67990-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HT-29 using 67990-1-Ig (same clone as 67990-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67990-1-Ig (MMP7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67990-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 67990-1-Ig (same clone as 67990-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 67990-1-Ig (MMP7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67990-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 67990-1-Ig (same clone as 67990-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 67990-1-Ig (MMP7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67990-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 67990-1-Ig (same clone as 67990-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67990-1-Ig (MMP7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67990-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 67990-1-Ig (same clone as 67990-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67990-1-Ig (MMP7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67990-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human pancreas cancer using 67990-1-Ig (same clone as 67990-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using MMP7 antibody (67990-1-Ig, Clone: 2E6D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67990-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human pancreas cancer using 67990-1-Ig (same clone as 67990-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using MMP7 antibody (67990-1-Ig, Clone: 2E6D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67990-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human pancreas cancer using 67990-1-Ig (same clone as 67990-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using MMP7 antibody (67990-1-Ig, Clone: 2E6D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67990-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50369-1

  Cytometric bead array standard curve of MP50369-1, MMP7 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67990-2-PBS. Detection antibody: 67990-1-PBS. Standard:Ag0550. Range: 3.125-100 ng/mL.

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Product Information

67990-1-PBS targets MMP7 as part of a matched antibody pair:

MP50369-1: 67990-2-PBS capture and 67990-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: MMP7 fusion protein Ag0550
• Full Name: matrix metallopeptidase 7 (matrilysin, uterine)
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 27-30 kDa
• GenBank Accession Number: BC003635
• Gene Symbol: MMP7
• Gene ID (NCBI): 4316
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.