MMP7 Monoclonal antibody

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  WB analysis of SKOV-3 using 67990-1-Ig

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 67990-1-Ig (MMP7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 67990-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 67990-1-Ig (MMP7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-29 using 67990-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67990-1-Ig (MMP7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 67990-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 67990-1-Ig (MMP7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 67990-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67990-1-Ig (MMP7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 67990-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67990-1-Ig (MMP7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human pancreas cancer using 67990-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using MMP7 antibody (67990-1-Ig, Clone: 2E6D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human pancreas cancer using 67990-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using MMP7 antibody (67990-1-Ig, Clone: 2E6D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human pancreas cancer using 67990-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using MMP7 antibody (67990-1-Ig, Clone: 2E6D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of PC-3 using 67990-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using MMP7 antibody (67990-1-Ig, Clone: 2E6D6 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

MMP7 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Cat No. 67990-1-Ig

Clone No.2E6D6

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

Matrix metalloproteinase-7, Matrix metalloproteinase 7, Matrin, Matrilysin, EC:3.4.24.23

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Tested Applications

• Positive WB detected in: SKOV-3 cells, HT-29 cells, COLO 320 cells, A549 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human pancreas cancer tissue
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67990-1-Ig targets MMP7 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: MMP7 fusion protein Ag0550
• Full Name: matrix metallopeptidase 7 (matrilysin, uterine)
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 27-30 kDa
• GenBank Accession Number: BC003635
• Gene Symbol: MMP7
• Gene ID (NCBI): 4316
• RRID: AB_2918739
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P09237
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.