MMP13 Recombinant antibody

MMP13 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240060A1

Cat no : 83188-4-RR

Synonyms

Matrix metalloproteinase-13, Matrix metalloproteinase 13, EC:3.4.24.-, Collagenase 3, CLG3

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Validation Data Gallery

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  WB analysis using 83188-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83188-4-RR (MMP13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  FC experiment of MCF-7 using 83188-4-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug MMP13 Recombinant antibody (83188-4-RR, Clone:240060A1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 83188-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83188-4-RR against Human MMP13 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: MCF-7 cells, MDA-MB-231 cells, PC-3 cells
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83188-4-RR is MMP13 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MMP13 fusion protein Ag34892
• Full Name: matrix metallopeptidase 13
• Calculated Molecular Weight: 471 aa, 54 kDa
• Observed Molecular Weight: 70 kDa, 54 kDa
• GenBank Accession Number: BC074808
• Gene Symbol: MMP13
• Gene ID (NCBI): 4322
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MMP-13(Matrix metalloproteinase-13), cleaves type I collagen and was previously thought to be the rodent analogue of human interstitial MMP-1. MMP-13 is found in hypertrophic and calcifying cartilage of mammalian growth plate. The bands seen with rat MMP-13 are consistent with a 59-kDa latent form and a 43-kDa active form reported for this enzyme, along with a smaller band at approximately 30 kDa(PMID:10771523). It also can be detected 3 bands that the glycosylated proMMP-13 (70 kDa), unglycosylated form (55 kDa), and active MMP-13 enzyme (40kDa) by western blot(PMID:18332263).