Neprilysin/CD10 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Neprilysin/CD10 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig, rabbit

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4B9D9

Cat no : 60034-3-PBS

Synonyms

MME, Neprilysin, Enkephalinase, EC:3.4.24.11, CD10

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Validation Data Gallery

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  WB analysis using 60034-3-Ig (same clone as 60034-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60034-3-PBS in a different storage buffer formulation.

• 

  WB analysis of Daudi using 60034-3-Ig (same clone as 60034-3-PBS)

  Daudi cells were subjected to SDS PAGE followed by western blot with 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60034-3-PBS in a different storage buffer formulation.

• 

  WB analysis using 60034-3-Ig (same clone as 60034-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60034-3-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 60034-3-Ig (same clone as 60034-3-PBS)

  WB result of Neprilysin/CD10 antibody (60034-3-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Neprilysin/CD10 transfected HEK-293 cells. This data was developed using the same antibody clone with 60034-3-PBS in a different storage buffer formulation.

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  WB analysis of Ramos using 60034-3-Ig (same clone as 60034-3-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60034-3-PBS in a different storage buffer formulation.

• 

  WB analysis of pig kidney using 60034-3-Ig (same clone as 60034-3-PBS)

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 60034-3-Ig (Neprilysin/CD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60034-3-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 60034-3-Ig (same clone as 60034-3-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 60034-3-Ig (Neprilysin/CD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60034-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 60034-3-Ig (same clone as 60034-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:50000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60034-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 60034-3-Ig (same clone as 60034-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:50000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60034-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 60034-3-Ig (same clone as 60034-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:100000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60034-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 60034-3-Ig (same clone as 60034-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:50000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60034-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 60034-3-Ig (same clone as 60034-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:50000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60034-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 60034-3-Ig (same clone as 60034-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:100000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60034-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human renal cell carcinoma using 60034-3-Ig (same clone as 60034-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:100000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60034-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 60034-3-Ig (same clone as 60034-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:50000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60034-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human renal cell carcinoma using 60034-3-Ig (same clone as 60034-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:100000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60034-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 60034-3-Ig (same clone as 60034-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:50000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60034-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsil using 60034-3-Ig (same clone as 60034-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsil using 60034-3-Ig(Neprilysin/CD10 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 60034-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsil using 60034-3-Ig (same clone as 60034-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsil using 60034-3-Ig(Neprilysin/CD10 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 60034-3-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 60034-3-Ig (same clone as 60034-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 60034-3-Ig (Neprilysin/CD10 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60034-3-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 60034-3-Ig (same clone as 60034-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 60034-3-Ig (Neprilysin/CD10 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60034-3-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 60034-3-Ig (same clone as 60034-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 60034-3-Ig (Neprilysin/CD10 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60034-3-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 60034-3-Ig (same clone as 60034-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 60034-3-Ig (Neprilysin/CD10 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60034-3-PBS in a different storage buffer formulation.

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Product Information

60034-3-PBS targets Neprilysin/CD10 in WB, IHC, ELISA applications and shows reactivity with human, mouse, pig, rabbit samples.

• Tested Reactivity: human, mouse, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Neprilysin/CD10 fusion protein Ag0427
• Full Name: membrane metallo-endopeptidase
• Calculated Molecular Weight: 750 aa, 86 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC101658
• Gene Symbol: CD10
• Gene ID (NCBI): 4311
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD10, also known as neprilysin, membrane metallo-endopeptidase (MME), neutral endopeptidase (NEP), or common acute lymphoblastic leukemia antigen (CALLA), is a 100-kDa type II transmembrane glycoprotein belonging to peptidase M13 family (PMID: 7760013; 8102558). Among hematopoietic cells, CD10 is expressed on granulocytes, B cell precursors, mature germinal center B cells, a subset of immature thymocytes (PMID: 13679451). CD10 is also expressed on a variety of nonhematopoietic cell types, including bronchial epithelial cells, cultured fibroblasts, bone marrow stromal cells, renal proximal tubular epithelial cells, breast myoepithelium, biliary canaliculi (PMID: 8102558). CD10 is a cell surface peptidase that cleaves peptide bonds on the amino side of hydrophobic amino acids and inactivates a variety of physiologically active peptides. Loss or decreases in CD10 expression have been reported in a variety of malignancies (PMID: 16054017).