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MLPH Recombinant antibody, PBS Only

MLPH Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241621G4

Cat no : 84282-1-PBS

Synonyms

SLAC2A, SLAC2 A, Melanophilin, Exophilin-3, Exophilin 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MLPH Recombinant antibody (84282-1-RR)

    WB analysis using 84282-1-RR (same clone as 84282-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84282-1-RR (MLPH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84282-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using MLPH Recombinant antibody (84282-1-RR)

    IHC staining of human skin cancer using 84282-1-RR (same clone as 84282-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84282-1-RR (MLPH antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84282-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using MLPH Recombinant antibody (84282-1-RR)

    IHC staining of human skin cancer using 84282-1-RR (same clone as 84282-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84282-1-RR (MLPH antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84282-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84282-1-RR

    Affinity and Kinetic Characterization of 84282-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84282-1-RR against Human MLPH were performed. The affinity constant is 2.43 nM.

Product Information

The immunogen of 84282-1-PBS is MLPH Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MLPH fusion protein Ag32931
Full Name melanophilin
Calculated Molecular Weight 66 kDa
Observed Molecular Weight66 kDa, 80 kDa
GenBank Accession NumberBC001653
Gene Symbol MLPH
Gene ID (NCBI) 79083
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Melanophilin (Mlph) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft.