MLKL Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

MLKL Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

3D4C6

Cat no : 66675-1-PBS

Synonyms

Mixed lineage kinase domain-like protein, hMLKL, 3D4C6

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Validation Data Gallery

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  WB analysis using 66675-1-Ig (same clone as 66675-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66675-1-Ig (MLKL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

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  WB analysis using 66675-1-Ig (same clone as 66675-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66675-1-Ig (MLKL antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 66675-1-Ig (same clone as 66675-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 66675-1-Ig (MLKL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66675-1-Ig (same clone as 66675-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66675-1-Ig (MLKL antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66675-1-Ig (same clone as 66675-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66675-1-Ig (MLKL antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66675-1-Ig (same clone as 66675-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66675-1-Ig (MLKL antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66675-1-Ig (same clone as 66675-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66675-1-Ig (MLKL antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66675-1-Ig (same clone as 66675-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66675-1-Ig (MLKL antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66675-1-Ig (same clone as 66675-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66675-1-Ig (MLKL antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 66675-1-Ig (same clone as 66675-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 66675-1-Ig (MLKL antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66675-1-Ig (same clone as 66675-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MLKL antibody (66675-1-Ig, Clone: 3D4C6 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

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Product Information

66675-1-PBS targets MLKL in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MLKL fusion protein Ag15188
• Full Name: mixed lineage kinase domain-like
• Calculated Molecular Weight: 471 aa, 54 kDa
• Observed Molecular Weight: 35-40 kDa, 50-55 kDa
• GenBank Accession Number: BC028141
• Gene Symbol: MLKL
• Gene ID (NCBI): 197259
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Mixed lineage kinase domain like pseudokinase (MLKL), belongs to the protein kinase superfamily and has two MW of 54 and 30 kDa. MLKL plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in the necroptosis pathway. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. The 66675-1-Ig antibody recognizes 54 kDa MLKL monomer, 216 kDa MLKL tetramer, and a band around 45-50 kDa which are similar to papers published. (PMID: 31848291). MLKL can be detected as a 35kDa truncated isoform (PMID: 35965541).