- Featured Product
- KD/KO Validated
MLKL Monoclonal antibody, PBS Only
MLKL Monoclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
3D4C6
Cat no : 66675-1-PBS
Synonyms
Validation Data Gallery
Recommended dilution
Application | Dilution |
---|---|
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. |
Product Information
66675-1-PBS targets MLKL in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse samples.
Tested Reactivity | Human, Mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | MLKL fusion protein Ag15188 |
Full Name | mixed lineage kinase domain-like |
Calculated Molecular Weight | 471 aa, 54 kDa |
Observed Molecular Weight | 35-40kDa, 50-55 kDa |
GenBank Accession Number | BC028141 |
Gene Symbol | MLKL |
Gene ID (NCBI) | 197259 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS only |
Storage Conditions | Store at -80°C. |
Background Information
Mixed lineage kinase domain like pseudokinase (MLKL), belongs to the protein kinase superfamily, has two MW of 54 and 30 kDa. MLKL plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a prograwmmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. 66675-1-Ig antibody recognizes 54 kDa MLKL monomer, 216 kDa MLKL tetramer and a band around 45-50 kDa which are similar to papers published. (PMID: 31848291). MLKL can be detected a 35kDa truncated isoform (PMID: 35965541).