MKNK2 Recombinant antibody, PBS Only (Detector)

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  IF Staining of MCF-7 using 83235-1-RR (same clone as 83235-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MKNK2 antibody (83235-1-RR, Clone: 240045B3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83235-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83235-1-RR (same clone as 83235-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MKNK2 Recombinant antibody (83235-1-RR, Clone:240045B3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83235-1-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 83235-1-RR (same clone as 83235-1-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug MKNK2 Recombinant antibody (83235-1-RR, Clone:240045B3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83235-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00216-2

  Cytometric bead array standard curve of MP00216-2, MKNK2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83235-4-PBS. Detection antibody: 83235-1-PBS. Standard: Ag10546. Range: 1.25-40 ng/mL.

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  Affinity and Kinetic Characterization of 83235-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83235-1-RR against Human MKNK2 were performed. The affinity constant is 75.1 pM.

MKNK2 {ptg:Brand} Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 83235-1-PBS

Clone No.240045B3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

MAPK signal-integrating kinase 2, MAP kinase-interacting serine/threonine-protein kinase 2, MAP kinase signal-integrating kinase 2, GPRK7, EC:2.7.11.1

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Product Information

83235-1-PBS targets MKNK2 as part of a matched antibody pair:

MP00216-2: 83235-4-PBS capture and 83235-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MKNK2 fusion protein Ag10546
• Full Name: MAP kinase interacting serine/threonine kinase 2
• Calculated Molecular Weight: 465 aa, 52 kDa
• Observed Molecular Weight: 47-52 kDa
• GenBank Accession Number: BC018345
• Gene Symbol: MKNK2
• Gene ID (NCBI): 2872
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9HBH9
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

MKNK2(MAP kinase-interacting serine/threonine-protein kinase 2) is also named as GPRK7, MNK2 and belongs to the CAMK Ser/Thr protein kinase family. It has a conserved C-terminal Erk-interacting domain, a catalytic domain with homology to the calcium/calmodulin-dependent family of kinases, and putative MAP kinase phosphorylation sites located within the T loop of the kinase domain(PMID:9155017). The breast cancer cells express either an uncharacterized form of MNK2 or a cross-reacting MNK2-like protein with the band of 72-75 kDa by western blot(PMID:17130135). MKNK2 has 2 isoforms with the molecular mass of 52 kDa and 47 kDa.