MITF Recombinant antibody, PBS Only (Detector)

MITF Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240815E7

Cat no : 83803-2-PBS

Synonyms

WS2A, Microphthalmia-associated transcription factor, MI, Class E basic helix-loop-helix protein 32, bHLHe32

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Validation Data Gallery

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  IF Staining of HepG2 using 83803-2-RR (same clone as 83803-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MITF antibody (83803-2-RR, Clone: 240815E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83803-2-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 83803-2-RR (same clone as 83803-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using MITF antibody (83803-2-RR, Clone: 240815E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83803-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 83803-2-RR (same clone as 83803-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MITF antibody (83803-2-RR, Clone: 240815E7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83803-2-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83803-2-RR (same clone as 83803-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MITF Recombinant antibody (83803-2-RR, Clone:240815E7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83803-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00746-1

  Cytometric bead array standard curve of MP00746-1, MITF Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83803-1-PBS. Detection antibody: 83803-2-PBS. Standard: Ag3679. Range: 0.781-100 ng/mL.

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  Cytometric bead array standard curve of MP00746-3

  Cytometric bead array standard curve of MP00746-3, MITF Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83803-3-PBS. Detection antibody: 83803-2-PBS. Standard: Ag3679. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 83803-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83803-2-RR against Human MITF were performed. The affinity constant is 31.9 pM.

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Product Information

83803-2-PBS targets MITF as part of a matched antibody pair:

MP00746-1: 83803-1-PBS capture and 83803-2-PBS detection (validated in Cytometric bead array)

MP00746-3: 83803-3-PBS capture and 83803-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MITF fusion protein Ag3679
• Full Name: microphthalmia-associated transcription factor
• Calculated Molecular Weight: 91 aa, 10 kDa, 59 kDa
• Observed Molecular Weight: 60-65 kDa
• GenBank Accession Number: BC012503
• Gene Symbol: MITF
• Gene ID (NCBI): 4286
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The retinal pigment epithelium (RPE) has a essential role in maintaining visual function and dedifferentiation of RPE contributes to the pathophysiology of several ocular diseases (PMID:22523078). Microphthalmia-associated transcription factor (MITF) is a key regulator of RPE differentiation that is also down-regulated in dedifferentiated hfRPE cells. MITF is a basic helix-loop-helix (hHLH)-leucine zipper protein that involves in the development of various cell types, including neural crest-derived melanocytes and optic cup-derived retinal pigment epithelial cells (PMID: 10578055).