MIOS Recombinant antibody

MIOS Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240832B9

Cat no : 83856-5-RR

Synonyms

Missing oocyte meiosis regulator homolog, GATOR2 complex protein MIOS, 240832B9

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Validation Data Gallery

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  WB analysis using 83856-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83856-5-RR (MIOS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83856-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83856-5-RR against Human MIOS were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: K-562 cells, HeLa cells, HEK-293T cells, Jurkat cells, NIH/3T3 cells, mouse brain tissue,

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83856-5-RR is MIOS Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MIOS fusion protein Ag15148
• Full Name: missing oocyte, meiosis regulator, homolog (Drosophila)
• Calculated Molecular Weight: 875 aa, 99 kDa
• Observed Molecular Weight: 82 kDa
• GenBank Accession Number: BC005883
• Gene Symbol: MIOS
• Gene ID (NCBI): 54468
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The mechanistic target of rapamycin complex 1 (mTORC1) controls growth by regulating anabolic and catabolic processes in response to environmental cues, including nutrients. Amino acids signal to mTORC1 through the Rag GTPases, which are regulated by several protein complexes, including GATOR1 and GATOR2. GATOR2, which has five components (WDR24, MIOS, WDR59, SEH1L, SEC13), is required for amino acids to activate mTORC1. PMID: 35831510