MIG6; ERRFI1 Recombinant antibody

MIG6; ERRFI1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240447C3

Cat no : 83475-2-RR

Synonyms

ERRFI1, 240447C3, MIG-6, MIG6, MIG 6

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Validation Data Gallery

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  WB analysis using 83475-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83475-2-RR (MIG6; ERRFI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HepG2 using 83475-2-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug MIG6; ERRFI1 Recombinant antibody (83475-2-RR, Clone:240447C3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 83475-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83475-2-RR against Human MIG6; ERRFI1 were performed. The affinity constant is 2.04 nM.

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Tested Applications

• Positive WB detected in: HCT 116 cells, HepG2
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83475-2-RR is MIG6; ERRFI1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MIG6; ERRFI1 fusion protein Ag33341
• Full Name: ERBB receptor feedback inhibitor 1
• Calculated Molecular Weight: 51kd
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: NM_018948
• Gene Symbol: MIG6
• Gene ID (NCBI): 54206
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ERRFI1 (the product of mitogen-inducible gene 6, also known as MIG6) is an early response gene encoding a non-kinase scaffold adaptor protein induced by various stimuli such as hormones and stresses. ERRFI1 is considered a negative regulator of EGFR because it can directly bind to EGFR, inhibit the catalytic activity of EGFR, and mediate EGFR lysosomal degradation. ERRFI1 may play important roles in regulating stress response, maintaining homeostasis in tissues like joints or cardiac muscle, and functioning as a tumor suppressor.