Tested Applications
Positive WB detected in | HCT 116 cells, HepG2 |
Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
83475-2-RR targets MIG6; ERRFI1 in WB, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | MIG6; ERRFI1 fusion protein Ag33341 Predict reactive species |
Full Name | ERBB receptor feedback inhibitor 1 |
Calculated Molecular Weight | 51kd |
Observed Molecular Weight | 51 kDa |
GenBank Accession Number | NM_018948 |
Gene Symbol | MIG6 |
Gene ID (NCBI) | 54206 |
RRID | AB_3671103 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q9UJM3 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ERRFI1 (the product of mitogen-inducible gene 6, also known as MIG6) is an early response gene encoding a non-kinase scaffold adaptor protein induced by various stimuli such as hormones and stresses. ERRFI1 is considered a negative regulator of EGFR because it can directly bind to EGFR, inhibit the catalytic activity of EGFR, and mediate EGFR lysosomal degradation. ERRFI1 may play important roles in regulating stress response, maintaining homeostasis in tissues like joints or cardiac muscle, and functioning as a tumor suppressor.
Protocols
Product Specific Protocols | |
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WB protocol for MIG6; ERRFI1 antibody 83475-2-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |