MIG6; ERRFI1 Recombinant antibody, PBS Only (Detector)

MIG6; ERRFI1 Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240447C3

Cat no : 83475-2-PBS

Synonyms

ERRFI1, 240447C3, MIG-6, MIG6, MIG 6

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Validation Data Gallery

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  WB analysis using 83475-2-RR (same clone as 83475-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83475-2-RR (MIG6; ERRFI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83475-2-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 83475-2-RR (same clone as 83475-2-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug MIG6; ERRFI1 Recombinant antibody (83475-2-RR, Clone:240447C3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83475-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00464-3

  Cytometric bead array standard curve of MP00464-3, MIG6; ERRFI1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83475-1-PBS. Detection antibody: 83475-2-PBS. Standard: Ag33341. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 83475-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83475-2-RR against Human MIG6; ERRFI1 were performed. The affinity constant is 2.04 nM.

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Product Information

83475-2-PBS targets MIG6; ERRFI1 as part of a matched antibody pair:

MP00464-3: 83475-1-PBS capture and 83475-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MIG6; ERRFI1 fusion protein Ag33341
• Full Name: ERBB receptor feedback inhibitor 1
• Calculated Molecular Weight: 51kd
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: NM_018948
• Gene Symbol: MIG6
• Gene ID (NCBI): 54206
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ERRFI1 (the product of mitogen-inducible gene 6, also known as MIG6) is an early response gene encoding a non-kinase scaffold adaptor protein induced by various stimuli such as hormones and stresses. ERRFI1 is considered a negative regulator of EGFR because it can directly bind to EGFR, inhibit the catalytic activity of EGFR, and mediate EGFR lysosomal degradation. ERRFI1 may play important roles in regulating stress response, maintaining homeostasis in tissues like joints or cardiac muscle, and functioning as a tumor suppressor.