CoraLite® Plus 555-conjugated MIF Polyclonal antibody

• Featured Product
• KD/KO Validated

MIF Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 555 Fluorescent Dye

Cat no : CL555-20415

Synonyms

GIF, GLIF, L dopachrome isomerase, L dopachrome tautomerase, MIF, MMIF, Phenylpyruvate tautomerase

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Validation Data Gallery

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  FC experiment of A549 using CL555-20415

  1X10^6 A549 cells were intracellularly stained with 0.2 ug CoraLite® Plus 555 Anti-Human MIF (CL555-20415) (red), or 0.2 ug CoraLite® Plus 555 Rabbit IgG Control Antibody(CL555-30000)(Blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC (Intra) detected in: A549 cells
• Positive FC detected in: A549 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-20415 targets MIF in FC (Intra) applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MIF fusion protein Ag14058
• Full Name: macrophage migration inhibitory factor (glycosylation-inhibiting factor)
• Calculated Molecular Weight: 115 aa, 12 kDa
• Observed Molecular Weight: 12 kDa
• GenBank Accession Number: BC000447
• Gene Symbol: MIF
• Gene ID (NCBI): 4282
• Conjugate: CoraLite® Plus 555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 554 nm / 570 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MIF is a pleiotropic cytokine that contributes to the pathogenesis of many autoimmune diseases through its upstream immunoregulatory function and its polymorphic genetic locus. MIF is a highly conserved protein of 12.5 kDa, with evolutionarily ancient homologues in plants, protozoans, nematodes, and invertebrates.